Document Details

Document Type : Article In Journal 
Document Title :
Identification and Genetic Characterization of Blastocystis hominis in Patients from Makkah, Saudi Arabia.
التعرف والتوصيف الجيني لمتبرعمة الكيسات البشرية في مرضى من مكة المكرمة ، المملكة العربية السعودية.
 
Subject : Scientific Research 
Document Language : English 
Abstract : Background: Blastocystis species (sp.) are gastrointestinal protozoan parasites with high prevalence rates worldwide. Blastocystis sp. show extensive genetic diversity with 17 different subtypes (STs) described to date. A few studies have investigated the prevalence and STs of Blastocystis sp. in Makkah, Saudi Arabia. Therefore, we aimed in this study to identify and characterize subtypes of Blastocystis sp. in the City of Makkah, Saudi Arabia. Methods: Stool samples were collected from 140 patients who presented to King Abdulaziz Hospital, Hera General Hospital and Modern Medical Center in Saudi Arabia. Different microscopic examination methods of patients’ stools and molecular analyses (using primers targeting SSU rRNA gene) were performed to identify and characterize STs of Blastocystis sp. Results: Our microscopic examination of stool samples showed that 96/140 patients (68.6%) had Blastocystis sp. infection. Clinical examination of infected patients revealed that 81 patients were symptomatic, whereas 15 were asymptomatic. Next, we isolated DNA from Blastocystis sp.-positive stool samples followed by PCR amplification of small-subunit ribosomal RNA (SSU rRNA) gene and sequence analysis. Our sequence analysis showed that subtype 3 (ST3) was the most prevalent (53.13%) followed by subtype 1 (ST1) (45.83%), whereas subtype 2 (ST2) was the least prevalent (1.04%). Moreover, our results showed that all three STs resulted in more symptomatic than asymptomatic cases. Finally, we identified novel haplotypes which comprised of 8 ST3, 6 ST1, and one ST2 haplotypes. Conclusion: Our identification of several haplotypes in patients’ stools confirms the genetic diversity of Blastocystis sp. and may explain the reported low host specificity and differential pathogenicity of Blastocystis sp. We believe that additional molecular epidemiological and genomic studies are needed to understand the prevalence and pathogenicity of different subtypes in humans and animal hosts. 
ISSN : 1178-6973 
Journal Name : Infection and Drug Resistance 
Volume : 2022 
Issue Number : 15 
Publishing Year : 1443 AH
2022 AD 
Article Type : Article 
Added Date : Wednesday, April 6, 2022 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ماجد ح واكدWakid, Majed H.InvestigatorDoctoratemwakid@kau.edu.sa
وعد ط الدهاسيAldahhasi, Waad TResearcherMaster 
Asmaa م القاضيEl-Kady, أسماء MResearcherDoctorate 
حاتم أ الشبراويElshabrawy, Hatem AResearcherDoctorate 

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