Document Details

Document Type : Article In Journal 
Document Title :
Rectangular coordination polymer nanoplates: Large-scale, rapid synthesis and their application as a fluorescent sensing platform for DNA detection
Rectangular coordination polymer nanoplates: Large-scale, rapid synthesis and their application as a fluorescent sensing platform for DNA detection
 
Subject : Chemistry 
Document Language : English 
Abstract : In this paper, we report on the large-scale, rapid synthesis of uniform rectangular coordination polymer nanoplates (RCPNs) assembled from Cu(II) and 4,4′-bipyridine for the first time. We further demonstrate that such RCPNs can be used as a very effective fluorescent sensing platform for multiple DNA detection with a detection limit as low as 30 pM and a high selectivity down to single-base mismatch. The DNA detection is accomplished by the following two steps: (1) RCPN binds dye-labeled single-stranded DNA (ssDNA) probe, which brings dye and RCPN into close proximity, leading to fluorescence quenching; (2) Specific hybridization of the probe with its target generates a double-stranded DNA (dsDNA) which detaches from RCPN, leading to fluorescence recovery. It suggests that this sensing system can well discriminate complementary and mismatched DNA sequences. The exact mechanism of fluorescence quenching involved is elucidated experimentally and its use in a human blood serum system is also demonstrated successfully. 
ISSN : 1932-6203 
Journal Name : PLoS One 
Volume : 7 
Issue Number : 1 
Publishing Year : 1433 AH
2012 AD 
Article Type : Article 
Added Date : Sunday, March 4, 2012 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Yingwei ZhangZhang, Yingwei ResearcherDoctorate 
Yonglan LuoLuo, Yonglan ResearcherDoctorate 
Jingqi TianTian, Jingqi ResearcherDoctorate 
عبدالله محمد عسيريAsiri, Abdullah MohamedResearcherDoctorateaasiri2@kau.edu.sa
عبدالرحمن عبيد اليوبيAl-Youbia, Abdulrahman ObaidResearcherDoctorateaalyoubi@kau.edu.sa
Xuping SunSun, Xuping ResearcherDoctoratesunxp@ciac.jl.cn

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